High-output sequencing kits produce ~400M read pairs, covering up to 20,000 single cell gene expression libraries at the suggested minimum depth of 20,000 read pairs/cell. Sequencing & Basic Data Processing: Libraries are sequenced on the NextSeq 500 sequencing system. The Core recommends providing at least 50,000 cells in suspension per sample, as fewer cells can make important QC steps difficult to accomplish. Multiple chips can be run on a single day. Each inlet can encapsulate as many as 10,000 cells or as few as 500 cells. Single-Cell Isolation & 10x Library Preparation: Each 10x chip is comprised of eight sample inlets into which single cell suspensions are loaded. Contact the Associate Director to arrange a consultation. After sequencing, the resulting barcoded sequences are fed into analysis pipelines that use the barcode information to map reads back to their original cell.ġ0x Genomics Chromium Single Cell ControllerįACS: Sample preparation via FACS can be completed by the Core. Barcoded products are pooled for downstream reactions to create short-read sequencer compatible libraries. Gel beads infused with millions of barcoded oligonucleotides are mixed with a sample and then added to an oil-surfactant solution to create gel beads in emulsion (GEMs). GemCode Technology provides a precisely engineered reagent delivery method that enables thousands of micro-reactions in parallel.
Simply provide a single cell suspension, and the Core will perform the services necessary to generate high quality sequencing data: cell sorting, library preparation, sequencing, and basic data analysis. The Advanced Analytics Core now offers turnkey 10x Genomics single cell services. Single cell library preparation (RNA-Seq, ATAC-Seq, etc…).
Single Cell Solutions (10x Genomics) 10x Genomics Single Cell Services:
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